A. CAPILLARY BLOOD COLLECTION
PRINCIPLE: SPECIMEN COLLECTION, THE SKIN
REQUIREMENT
·
SWABS
·
SURGICAL /METHYLATED SPIRIT
·
GLOVES
PROCEDURE
1.
Feel the finger of choice you want to prick , if
its cold massage it
2.
Swab with on sweep gently and observe the swab,
if dirty repeat the swab
3.
Drawing an imaginary v, prick outside the v with
one gendle steady prick
4.
Wipe the first drop
5.
And use the second drop for the next exercise
B. BLOOD GROUPING
CONCEPT:ANTIGEN ANTIBODY REACTION
·
REQUIREMENT
·
BLOOD GROUPING REAGENT
·
CAPILLARY BLOOD
·
SLIDES
PROCEDURE
1. One
drop 💧 of blood is
separately placed three times on a glass
slide
2. Starting
with anti A, placed on the first drop then anti B placed on the second drop
finally anti D placed
3. Using
the corner of another clean slide mix the blood and specimen making sure that
every corner of the slide is only used once in mixing
4. Pick
the slide with you just mixed containing the 3 drops of blood and blood
grouping reagents and shake gently ensuring that the contents don’t come closer
to each other
5. Read
and interpret your result’’
C.
RAPID
TESTS
CONCEPT :ANTIGEN ANTIBODY REACTION,
CHROMATRAPHICAL TECHNIQUE
REQUIREMENT
·
RAPID TEST KITS
·
SPECIMEN
·
BUFFER
PROCEDURE
1. Collect
the specimen of choice for the rapid test
2. Using
the collecting tool provided by the manufacturer place the sample in pot
3. Add
the buffer
4. Read
the result
5. Interpret
the results
D. STERILIZATION TECHNIQUE
Requirement
Autoclave
Items to be sterilized
Timer
PROCEDURE
1. Load the autoclave with deionized water to the
level of the stand
2. Wrap
instruments for autoclaving in sets
wrapped in the blue towel and label
3. Tie
a steam indicator strip into the wrap.
4. Make
sure you have locked airtight the autoclave lid and tighten the screw .Make
sure that the air valve is not released
5. Switch
on the autoclave
6. On
reaching 15psi, start documenting your time for 15 minutes
7. After
15 minutes switch off the autoclave and release the air valve
8. Wait
for the items to cool and remove them observing any changes in the
sterilization tape
9. If
the steam indicator tape, package markings or interior steam indicator strip do
not change color after processing the instruments are repackaged and
reprocessed.
E.
CULTURE
TECHNIQUES
1. To
demonstrate the presence of microorganisms RIVER WATER
2. Appreciate appearance of bacterial colonies on solid media
REQUIREMENTS
Autoclave
Incubator
Micropipette
Petri dishes
Culture media
Micropipette
Collection bottles
PROCEDURE
1. Autoclave
the petri dishes, swabs ,agar media prepared and collection bottles
2. Collect
the following samples Dam water down the college
3. Using
micropipette pipette 50micrlitre of the mixed samples collected above and place
the sample on culture media prepared and spread with the applicator sticks
4. Incubate for 24hrs ,48hrs and record your finding by taking pictures
and counting the number of colonies identified
VIDEO LINKS TO BE WATCHED BEFORE THE
SKILLS
https://www.youtube.com/watch?v=rS3iCNP8NRI Culture methods
https://www.youtube.com/watch?v=yVRj7CWFj9A capillary blood collection
https://www.youtube.com/watch?v=Lx3CAmMpvCE blood grouping
https://www.youtube.com/watch?v=j23s_cxwxSE sterilization techniques
https://www.youtube.com/watch?v=WqT2z8jCEHs rapid tests
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